Item# 1001-P: Peroxidase-Conjugated rgp120 HIV-1 IIIB
Concentration: See vial
Diluent: 50mM Tris, pH 8 1% BSA
Preservative: 0.01% TM
Stability: At least 6 months at 4°C
Minimum Dilution: 1 : 1000
Application: ELISA/Western ELISA, Drug Screening, Human T-cell Research, Histocytochemistry, CD4 Binding.
Description: Peroxidase conjugated rgp120 HIV-1 IIIB (Baculovirus).
Molar Ratio: 2 - 4 peroxidase molecules per molecule of gp120.
Specificity: Peroxidase conjugated rgp120 IIIB binds to murine monoclonal antibodies of defined epitope specificity and human monoclonal/polyclonal antibodies in ELISA and Western ELISA
Biological Activity: Peroxidase conjugated rgp120 IIIB binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA.
Application and Instructions for use
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA may be performed at dilutions of 10 : 100ug protein depending on the nature and affinity of the detection reagent. HIV- converted human serum polyclonal antibodies yield titers 1:1000 or greater at 1-10ug of immobilized protein under standard Elisa condition.