Product# 1081-B: Biotin rgp120 HIV-1 ADA
Mass/Vial: 50µg T-Cell Research
Vol./Vial: 50µl Diagnostics
Diluent: PBS Drug Screening
Stabilizer: 0.2% BSA ELISA, Western ELISA
Stability: Not Determined
Minimum Dilution: 1 : 2000
DESCRIPTION: Biotin conjugated to rgp120 ADA
COUPLING RATIO: Approximately 10-20 moles of biotin per mole gp120.
SPECIFICITY: Biotin gp120 ADA binds strepavidin-AP/HRP in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
- ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and .1-1.0ng of biotin-gp120.