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Product Specifications: Item# 1001-B: Biotin rgp120 HIV-1 IIIB Mass/Vial: 50µg Vol./Vial: 50ul Diluent: PBS+ 0.2% BSA, pH 7.5 Stabilizer: BSA Preservative: 0.1% NaN3 Storage: 4°C Stability: 6 Months Minimum Dilution:1 : 2,000 Application: T-Cell Research, Diagnostics, ELISA, Western ELISA, Drug Screening. Description: Biotin conjugated to rgp120 HIV-1 IIIB. Coupling Ratio: Approximately 5-20 moles of biotin per mole gp120. Specificity: Biotin gp120 binds to Murine and Human Anti-gp120 mAbs as determined by strepavidin-HRP ELISA and Western ELISA assays. Biological Activity: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 10,000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and 0.1-10ng of biotin-gp120. |
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