Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in saline-citrate buffer (150mM NaCl, 50mM sodium citrate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR dependent transcriptional activation activity.
Transcriptional activation assays with tat are performed in 1-5μg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.
Related HIV-1 tat products: Item #1032 tat HIV-1 MN , Item #1052 tat HIV-1 BAL and mutant bal