Product# 1081 HIV-1 gp120 (ADA)
Product# 1011 HIV-1 gp120 (subtype C)
Product# 1031 HIV-1 gp120 (YU2)
Monocytes and monocyte–derived macrophages express relatively low levels of CD4. Despite this, macrophages can be effectively infected with human immunodeficiency virus type 1. Macrophages have a critical role in human immunodeficiency virus type 1 transmission; however, the mechanism or mechanisms of virus infection are poorly understood. We report that growth factors, such as granulocyte macrophage colony-stimulating factor and macrophage colony-stimulating factor affect the phenotypic profile and permissiveness of macrophages to human immunodeficiency virus type 1. Human immunodeficiency virus type 1 infection of monocyte–derived macrophages derived from granulocyte macrophage and macrophage colony-stimulating factors was predominantly facilitated by the sialic acid-binding immunoglobulin-like lectin-1. The number of sialic acid-binding immunoglobulin-like lectin receptors on macrophage colony-stimulating factor–derived monocyte–derived macrophages was significantly greater than on granulocyte macrophage colony-stimulating factor–derived monocyte–derived macrophages, and correspondingly, human immunodeficiency virus type 1 infection was greater in the macrophage colony-stimulating factor–derived monocyte–derived macrophages. Single-genome analysis and quantitative reverse transcriptase-polymerase chain reaction revealed that the differences in infectivity was not due to differences in viral fitness or in viral variants with differential infectivity but was due to reduced viral entry into the granulocyte macrophage colony-stimulating factor–derived monocyte–derived macrophages. Anti-sialic acid-binding immunoglobulin-like lectin, trimeric glycoprotein 145, and scaffolded V1V2 proteins were bound to sialic acid-binding immunoglobulin-like lectin and significantly reduced human immunodeficiency virus type 1 entry and infection. Furthermore, sialic acid residues present in the V1V2 region of the envelope protein mediated human immunodeficiency virus type 1 interaction with sialic acid-binding immunoglobulin-like lectin and entry into macrophage colony-stimulating factor–derived monocyte–derived macrophages. Removal of sialic acid residues or glycans from scaffolded V1V2 protein decreased human immunodeficiency virus type 1 infectivity. These results highlight the importance of sialic acids on the V1V2 region in binding to sialic acid-binding immunoglobulin-like lectin and suggest that the unusually long surface-exposed sialic acid-binding immunoglobulin-like lectin might aid in the capture and entry of human immunodeficiency virus type 1 into monocyte–derived macrophages.
Keywords: GM-CSF, M-CSF, qRT-PCR, viral entry, flow cytometry, surface plasmon resonance
Macrophages are targets of HIV-1 and represent a potentially long-lived viral reservoir. In humans, macrophages arise from circulating or resident monocytes, and their differentiation and effector functions are largely dependent on the surrounding microenvironment [1, 2]. Generally, macrophages are classified into 2 types, classically activated (M1) and alternatively activated (M2) macrophages. Following in vitro stimulation with various stimuli, M2 macrophages are further divided into 3 subtypes (M2a, M2b, and M2c) . Macrophages exhibit phenotypic heterogeneity that is dependent upon the cytokines present within their environment [4, 5]. GM-CSF and M-CSF are macrophage growth factors that have distinct effects on macrophage differentiation, resulting in either the M1 or the M2 phenotype . M1 macrophages produce IL-1β, IL-12, IL-23, and TNF-α, as well as reactive oxygen and nitrogen intermediates; support Th1 responses; and mediate resistance to tumors and intracellular pathogens. M2 macrophages secrete IL-10, express scavenger and mannose receptors, contribute to Th2 responses, enhance phagocytosis, eliminate parasites, and promote tissue repair [7, 8].
CD4 molecules and chemokine receptors are the major receptor/coreceptors used by HIV-1 for infection of target cells. However, unlike CD4+ T cells, macrophages and MDMs express relatively low levels of surface CD4, thus, confounding the role of this molecule in HIV-1 entry in macrophages. Other unique cellular molecules, such as α4β7 [9, 10], tetraspanins , and heparan sulfate proteoglycans , have a role in HIV-1 infection. Recently, Siglec-1 was shown to facilitate HIV-1 infection of DCs [13, 14] and macrophages  by binding to the sialoglycans on the gp120 envelope. To date, 15 different Siglec receptors have been characterized in humans, and these proteins specifically recognize the terminal SAs associated with both N- and O-linked glycosylation promoting cell-to-cell adhesion . The viral envelope of HIV is heavily glycosylated, and SAs on the viral envelope interact with Siglec receptors, in particular Siglec-1 (CD169), on macrophages and DCs, facilitating HIV-1 infection. Siglec-1 on DCs capture HIV-1 by interacting with sialyllactose-containing gangliosides exposed on viral membranes and subsequently mediate trans-infection of CD4+ T cells [13, 17]. In the case of macrophages, it is not known which region of the HIV-1 envelope protein interacts with Siglec-1 or whether the conformation of the viral envelope protein is also important in that interaction.
In the present study, we evaluated the effects of GM-CSF and M-CSF on the expression of Siglec receptors on primary human MDMs, the relationship between Siglec receptors and the permissiveness of HIV-1 infection, and the interaction of Siglec-1 with HIV-1 trimeric envelope protein. We further investigated whether the V1V2 region of HIV-1 gp120 protein interacted with Siglec-1 and how that interaction influenced HIV-1 infectivity.
MATERIALS AND METHODS
RV229B (WRAIR protocol 1386) “Apheresis of blood components from healthy volunteers for in vitro research” and all related documents were approved by the following independent institutional review boards: the Division of Human Subject Protection, the WRAIR, and the Ethical Review Committee for Research in Human Subjects. All volunteers were provided written, informed consent after discussion and counseling by the clinical study team before enrollment and before any blood was drawn.
The human mAbs anti-CD1a APC (clone HI149), CD11b PE (clone ICRF44), CD11b FITC (clone ICRF44), CD4 PE (clone RPA-T4), CD4 purified (clone RPA-T4), Siglec-3 (CD33)-APC (clone WM53), CD206 APC (clone 19.2), CD14 APC (clone M5E2), CD14 PerCP (clone MoP9), CD33 purified (clone WM53), CD4 PE (clone SK3), CD4 purified (SK3), CD195 PE (clone 2D7/CCR5), CD195 purified (clone 2D7/CCR5), HLA-A,B,C (clone G46-2.6), and 7-amino actinomycin D were obtained from BD Pharmingen (San Jose, CA USA). Purified Siglec-1, Siglec-5, and Siglec-9 mAbs, and the corresponding Siglec mAbs with the fluorochromes anti-Siglec-1 (CD169)-APC (clone 7-239), Siglec–9 PE (clone K8), and Siglec-5 (CD170)-PE (clone 1A5), were obtained from BioLegend (San Diego, CA, USA). Anti-HLA-DR (clone L203) was obtained from R&D Systems (Minneapolis, MN, USA). Anti-p24-FITC and anti-p24-RD1 were purchased from Beckman Coulter (Indianapolis, IN, USA).
Media and reagents
Media components and reagents were obtained as follows: RPMI 1640; l-glutamine, penicillin/streptomycin, and EDTA (Quality Biologicals Inc., Gaithersburg, MD, USA); Accutase (eBioscience, San Diego, CA, USA); recombinant human M-CSF and GM-CSF (PeproTech, Inc, Rocky Hill, NJ, USA); and FBS (Gemini Bio Products, West Sacramento, CA, USA). BSA, Nonidet P-40, Polybrene, Triton X-100, SDS, dual 5′ FAM-labeled and 3′ TAMRA-labeled probes and amplifying primers, and dual 5′ FAM were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fixation and permeabilization buffer reagents A and B were from Life Technologies (Frederick, MD). Proteinase K, 2X GeneAMP Fast PCR Master Mix, 2X TaqMan Universal PCR Master Mix, and dual 5′ VIC-labeled and 3′ TAMRA-labeled probes were purchased from Applied Biosystems (Foster City, CA, USA). Tris-HCl and Tween-20 were purchased from Invitrogen (Carlsbad, CA, USA).
Cyclic peptides were synthesized by JPT Peptide Technologies (Berlin, Germany). Peptides were cyclized by disulfide bond formation, and the purity was determined to be >90% by HPLC and mass spectrometry. The amino acid sequence of cyclic V2 peptides was based on vCP1521 envelope gp of HIV-1 CRF01 AE (92TH023 strain), GenBank accession number EF553537.1, and has been previously described .
Construction, expression, and purification of recombinant HIV-1 envelope proteins
JR-FL and SF162 gp120 and trimeric gp145 were produced in 293F cells. V1V2 proteins and CHO-S cells were also produced in these cells as either Soc or as gp16-scaffolded proteins. Soc is a nonessential Soc of bacteriophage T4, and gp16 is an 18-kDa oligomeric complex required for packing T4 DNA .
Soc and T4 gp16 were codon-optimized using GeneArt gene synthesis by Life Technologies (Carlsbad, CA, USA). In the N terminus, a GLuc secretion signal from Gaussia luciferase protein was included to allow for sufficient protein secretion, and a hexahistidine tag was included in the C terminus to facilitate protein purification. The V1V2 loop of SF162, JR-FL, and the transmitted/founder virus Zm249 were constructed using gene assembly PCR . Restriction sites BamHI and EcoRI were included in the N and C terminus of the V1V2 loop, respectively. The fragments were then cloned into a linearized vector containing either a GLuc-RB69soc-BamHI-EcoRI-hexahistadine tag or a GLuc-T4gp16-BamHI-EcoRI-hexahistadine tag. JR-FL and SF162 gp145 DNA were provided by Dr. Peter Kwong (National Institutes of Health, Bethesda, MD, USA). The codon-optimized constructs contain a CD5 secretion signal, complete gp120 and gp41 ectodomains, a mutation in the proteolytic cleavage site, and a trimerization domain from bacteriophage T4 fibritin  in the C terminus. JR-FL and SF162 gp120 were constructed from gp145 clones using PCR. All constructs were ligated with the linearized and dephosphorylated pcDNA3.1 vector (Invitrogen).
Transient transfection was performed using linear polyethylenimine (PEI25k, Polysciences, Inc., Warrington, PA, USA). Briefly, suspension cells 293F and CHO-S were grown per manufacture’s suggestions to maintain cells in their exponential growth phase. Two hours before transfection, the cell suspension was centrifuged (100 g for 5 min) and resuspended in fresh medium at 3 × 106 cell/ml. Cells were then transfected with 1 μg DNA/106 cells at a polyethylenimine to DNA ratio of 3:1. The following day, media was added to the cells to bring the cell concentration to 1 × 106 cell/ml. Transfection supernatants were harvested on day 3 and clarified using a 0.2-μm filter.
The clarified supernatant was loaded onto a HisTrap column (GE Healthcare, Little Chalfont, United Kingdom) and washed with buffer containing 20 mM imidazole. The protein was eluted with a 20–300-mM linear imidazole gradient. Soc–V1V2 loop peak fractions were concentrated using a 10-kDa molecular weight cutoff Amicon Ultra-4 centrifugal filter unit (Millipore, Billerica, MA, USA), and buffer was exchanged in PBS (pH 7.4) and stored at −80°C. The gp145 and gp120 peak fractions were concentrated and applied to a Hi-Load 16/60 Superdex-200 (preparation grade) gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-HCl (pH 8.0) and 100 mM NaCl. The peaks corresponding to a trimeric gp145 and monomeric gp120 were identified, pooled, concentrated, and stored at −80°C.
Desialylation and deglycosylation of Soc–V1V2
Purified Soc–V1V2 Zm249 scaffold protein (10 µg) was desialylated by treatment with 60 units of α2-3,6,8,9 neuraminidase A in 50 mM sodium acetate buffer (pH 5.5) containing 5 mM CaCl2 or deglycosylated with 1500 units of PNGase F (New England BioLabs Inc., Ipswich, MA, USA) in 50 mM sodium phosphate buffer (pH 7.5). The samples were incubated for 5 h at 37°C. The control samples lacked the neuraminidase A or PNGase F treatment.
To remove the enzymes, the treated and control samples were incubated with Ni-NTA agarose beads (Qiagen, Valencia, CA, USA) and placed on a rotating platform for 3 h at 4°C. The bead mixture was then spun down in a Pierce spin column (Thermo Fisher Scientific, Rockford, IL, USA), and the flow-through fraction was collected. The beads captured the His-tagged Soc–V1V2 protein, whereas the free neuraminidase A and PNGase F enzymes eluted in the flow-through fraction. To remove any residual enzyme, the beads were washed twice with an excess of 50 mM Tris-HCl buffer (pH 8.0), containing 300 mM NaCl. Bound Soc–V1V2 proteins were then eluted with 300 mM imidazole, concentrated, and buffer exchanged (25 mM Tris-HCl buffer [pH 8.0] containing100 mM NaCl) using 10-kDa MW cutoff Amicon Ultra-4 centrifugal filter units (Millipore).
Untreated neuraminidase A and PNGase F–treated Soc–V1V2 Zm249 scaffold proteins, before and after removal of the respective enzymes from the reaction mixture, were run on a 4–20% gradient Tris-glycine gel (Life Technologies) under reducing conditions. The gels were stained with Coomassie brilliant blue. Treatment with neuraminidase A or with PNGase F resulted in desialyation and deglycosylation as visualized by a shift in the mobility of the protein on SDS-PAGE with the proteins showing sharper bands after enzymatic treatment compared with the diffused bands before treatment. Neuraminidase A and PNGase F were removed from the reaction mixtures by using Ni-NTA agarose beads, which specifically bound to His-tagged Soc–V1V2, allowing the enzymes to pass in the flow-through fractions.
Virus stocks of US-1 and BaL (HIV-1 primary isolates subtype B), MO66 (primary isolate subtype CRF01_AE), and TZBD9/11 (primary isolates subtype C) grown in PBMCs were provided by Dr. Victoria Polonis (U.S. Military HIV Research Program, Silver Spring, MD, USA). The viruses were purified as previously described . Infectivity and p24 concentrations were determined before and after purification to ensure that infectivity was not lost during the purification procedure.
Enrichment and in vitro culture of monocytes
PBMCs were isolated by Ficoll density gradient centrifugation from healthy HIV-1–seronegative donors under an internal review board–approved protocol (RV229/WRAIR 1386). Monocytes were enriched from the PBMCs by plastic adherence. Briefly, PBMCs were resuspended in serum-free monocyte attachment media (RPMI 1640 supplemented with 1% l-glutamine and 1% penicillin/streptomycin), transferred to a tissue culture flask (CoStar Group, Washington DC, USA), and incubated at 37°C/5% CO2 for 1 h. The nonadherent cells were aspirated, and the monolayer was washed with monocyte media (RPMI 1640 supplemented with 10% heat-inactivated FBS, 1% l-glutamine, and 1% penicillin/streptomycin) to remove the remaining nonadherent cells. Accutase was added and the flask was incubated at 37°C/5% CO2 for 30 min. Most of the cells are detached at that time, and gentle pipetting detached the remaining cells. Cells were washed, counted, and analyzed by flow cytometry or plated in 24-well plates. Routine flow cytometric analysis with anti-CD14, anti-CD3, anti-CD11b, and 7-amino actinomycin D for monocyte characterization showed a purity of >90% and viabilities of >98%, with no T cell contamination. Similar viabilities were also observed with trypan blue exclusion. The enriched monocytes were subsequently differentiated into MDMs in monocyte medium supplemented with M-CSF or GM-CSF medium. Both growth factors were used at a final concentration of 50 ng/ml. For evaluation of surface markers, MDMs were harvested on days 1, 3, and 5 postculture; stained; and analyzed by flow cytometry. For infection with HIV-1, the MDMs were used on day 5 postculture.
HIV-1 infection of MDMs
MDMs differentiated in M-CSF medium or in GM-CSF medium were plated at 0.5 × 106 cells per well in 24-well plates (CoStar), as described above, and infected with HIV-1 as previously described . Briefly, the cells were incubated with 300 μl of infection media (monocyte medium containing 2 μg/ml Polybrene) at 37°C/5% CO2 for 30 min. Following aspiration, 300 μl of fresh infection medium containing purified HIV-1 (5 ng p24/300 μl) or envelope-deficient HIV-1 (pSG3Δenv) (5 ng p24/300 μl) was added to each monolayer, and the plate was centrifuged at 2500 rpm for 90 min at 37°C. pSG3Δenv contained a 4-nucleotide insertion mutation (CTAG) in the envelope, leading to a translation stop codon after 142 aa residue. The pSG3Δenv clone is routinely used for generating envelope pseudo-typed infectious virions. Unadsorbed virus was removed, and the cells were incubated at 37°C/5% CO2 in 1 ml of M-CSF or GM-CSF medium containing 2 μg/ml of Polybrene. Culture supernatants and cells were harvested at predetermined time points (15 and 30 min and 1, 3, 24, and 96 h postinfection for entry assays; 2, 4, and 10 d postinfection for infectivity assays). Supernatants were stored at −20°C. Cells were harvested and stained, and the percentage of HIV-1–infected cells (p24+ MDMs) was determined by flow cytometry.
Detection of cell-surface molecules
MDMs were harvested and washed in cold FACS buffer (PBS containing 0.5% BSA). The cells were incubated for 20 min at 4°C with a cocktail containing 5–10 μg of the specific mAb or its corresponding isotype controls. Cells were washed in cold FACS buffer and fixed in PBS containing 2% paraformaldehyde. Four-color flow cytometry was performed on a FACSCalibur cell analyzer (BD Biosciences). Data analyses were performed on the gated CD14+ cells using FlowJo 8.8.6 software (Tree Star Inc.).
Detection of intracellular HIV-1 p24 antigen
Briefly, HIV-1-infected and uninfected MDM were resuspended in cold FACS buffer (PBS-containing 0.5% BSA) and incubated with 5-10 μg anti-CD14 mAb for 20 min at 4°C. Cells were washed in cold FACS buffer and fixed in 100 μl Reagent A, in the dark, for 15 min at room temperature. Following washing in FACS buffer, the cells were permeabilized with 100 μl Reagent B and stained with anti p24-FITC or anti p24-PE for 15 min at room temperature. Cells were washed in cold FACS buffer and resuspended in PBS. Flow cytometry was performed on a FACSCalibur system. Data analyses were performed on the gated CD14+ cells using FlowJo 8.8.6 software (Tree Star, Ashland, OR, USA).
Quantification of cell-surface receptors
The number of Siglec-1, CCR5, or CD4 receptors/cell was determined using Quantum Simply Cellular Beads (Bangs Laboratories, Inc., Fishers, IN, USA) according to the manufacturer’s instructions. Briefly, MDMs (0.5 × 105 cells/tube) were preincubated with 10% normal goat sera, followed by the addition of a mAb cocktail of anti-human Siglec-1 (CD169)–APC, anti-human CCR5 (CD195)–FITC, or anti-human CD4-PE for 30 min at 4°C. Cells were then fixed with formaldehyde. Beads from the kit were stained with anti-human Siglec-1 (CD169)–APC, anti-human CCR5 (CD195)–FITC, or anti-human CD4-PE. Individual standard curves were established using the stained beads. Samples were acquired on a FACSCalibur system and analyzed with FlowJo 8.8.6 software. Data were placed into QuickCal version 2.3 software (Bangs Laboratories), and the number of Siglec-1, CCR5, or CD4 receptors/cell was extrapolated from the standard curves generated with the stained beads.
M-CSF–derived or GM-CSF–derived MDMs were plated at 0.5 × 106 cells/well in 24-well plates. The cells were preincubated with 300 μl of purified mAbs (10 μg/ml), N-acetylneuraminic acid (10 mM SA; Carbosynth, Compton, West Berkshire, United Kingdom), lactose (50 mM), sialyllactose (GM3, 10 mM), recombinant-scaffolded HIV-1–V1V2 proteins (20 μg/ml), PNGase-treated recombinant HIV-1–V1V2 proteins (20 μg/ml), neuraminidase A–treated recombinant HIV-1–V1V2 proteins (20 μg/ml), or varying concentrations of JR-FL FD gp145 trimer for 30 min at room temperature. Following aspiration and washing, 300 μl of fresh infection medium containing purified HIV-1 was added to each monolayer, and the plate was centrifuged at 2500 rpm for 15 or 30 min at 37°C (for HIV-1 entry experiments) or for 90 min at 37°C (for HIV-1 replication experiments). For HIV-1 entry experiments, unadsorbed virus was removed, and the cells were washed 3 times with PBS to remove residual virus. The cells were subsequently lysed, and HIV-1 entry was determined by qRT-PCR or by qPCR. For HIV-1 replication, unabsorbed virus was removed, and the cells were incubated at 37°C/5% CO2 in 1 ml of M-CSF or GM-CSF medium containing Polybrene. Cells were harvested and stained, and the percentage of HIV-1–infected cells was determined by flow cytometry.
Surface plasmon measurements were conducted with a Biacore T200 (GE Healthcare). Siglec-1 was immobilized onto a CM5 sensor chip using a standard amine-coupling method. Escherichia coli expressed recombinant T4gp15 protein or Fc–mucosal addressin cell adhesion molecule-1 protein was immobilized on the reference flow cell. Briefly, the chip surface was activated by a 1:1 mixture of 0.4 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 0.1 M N-hydroxysuccinimide for 7–10 min with a flow rate of 10 µl/min. Siglec-1 (2500 RUs) and T4gp15 (3100 RUs) were coupled. The immobilized surface was deactivated with 1.0 M ethanolamine–HCl (pH 8.5) for 10 min and stabilized with multiple injections of 0.05% SDS and 50 mM HCl. In separate experiments, JR-FL monomeric gp120 (subtype B, 900 RUs), JR-FL FD trimeric gp145 (1400 RUs), SF162 FD trimeric gp145 (subtype B, 900 RUs), Soc–V1V2JR–FL (1150 and 8000 RUs), and Soc–V1V2SF162 (1150 RUs) were immobilized on different CM5 chips using the same standard amine-coupling procedure described above.
Varying concentrations (0.98–1000 nM) of gp120, gp145, and Soc–V1V2 proteins, or alternatively, Siglec-1 in 10 mM HEPES and 150 mM NaCl (pH 7.4) were injected over immobilized Siglec-1 or gp120/gp145/Soc–V1V2 proteins, respectively, at a flow rate of 30 µl/min, for 2 min, followed by a dissociation time of 8 min. The chip surface was regenerated with 0.05–0.075% SDS for 30–45 s. Each kinetic assay was performed in triplicate. The data were analyzed using the BIAevaluation 4.1 software (GE Healthcare). The data were fitted into a bivalent model (2 binding sites on 1 analyte molecule bind to 2 ligand molecules) after subtraction of the RU values obtained with the reference flow cell and the buffer. A bivalent analyte gives rise to 2 sets of rate constants following the reaction equations A + B ↔ AB (k-forward = 2 × ka1; k-backward = kd1; KD1 = kd1/ka1) and AB + B ↔ AB2 (k-forward = ka2; k-backward = 2 × kd2; KD2 = kd2/ka2), where A is an analyte and B is a ligand. Parameters, including ka (ka1 and ka2), kd (kd1 and kd2), Rmax, and tc, were fitted globally, whereas RI was fitted locally, and concentration was fitted as a constant with bivalent binding model. Mass transfer and the closeness of the fit were assessed by tc and χ2 values, respectively.
Competition binding assays were performed out in triplicate with varying concentrations (1–100 mM) of lactose (negative control), 6’-SL (Carbosynth), and SA (Carbosynth). Briefly, 50–200 nM gp120/gp145/Soc–V1V2 or 50 nM Siglec-1 were injected in the presence or absence of lactose, 6′-SL, or SA onto immobilized Siglec-1 or gp120/gp145/Soc-V2 proteins using similar buffer conditions as mentioned above. The data analysis was performed with BIAevaluation 4.1 software, as mentioned above.
RNA extraction and qRT-PCR for viral entry
RNA was extracted from GM-CSF–derived or M-CSF–derived MDMs infected with HIV-1 (BaL, subtype B) using the RNeasy Mini Kit and QIAshredder (Qiagen). The RNA was eluted in RNase free water, and the concentration was determined with a Nanodrop 2000 (Thermo Scientific). The qRT-PCR reactions were performed using the TaqMan RNA-to-Ct Master Mix and the Viia7. Reactions (50 μl) were performed in the presence of the master mix, 0.2 μM each of gag forward and reverse primers (5′-CATGTTTTCAGCATTATCAGAAGGA-3′; 5′-TGCTTGATGTCCCCCCACT-3′), gag probe (5′-6FAM- CCACCCCACAAGATTTAAACACCATGCTAA-TAMRA-3′), and 1X human GAPDH VIC-TAMRA. Cycling parameters were 48°C for 20 min, 95°C for 10 min, then, 45 cycles at 95°C for 15 s, and 59°C for 1 min. ΔCt values were calculated to normalize the HIV gag level as a function of the GAPDH/cellular RNA signal. When comparing the GM-CSF–derived vs. the M-CSF–derived MDMs for viral entry, the ΔΔCt values were determined by ΔΔCt = (ΔCt of GM-CSF sample) – (ΔCt of M-CSF sample). The percentage of inhibition at entry was based on comparative qPCR analysis in the presence of the inhibitor vs. in the absence of the inhibitor (PBS). When examining the possible inhibitors of entry, the ΔΔCt values were determined by ΔΔCt = (ΔCt of PBS sample) – (ΔCt of inhibitor sample). Fold-difference was calculated with 2−∆∆Ct. The percentage of inhibition was calculated based on the equation −([Fold-difference] − 1) × 100.
DNA extraction and qPCR
DNA from 0.15–1.5 × 106 GM-CSF–derived or M-CSF–derived MDMs infected with HIV-1 (BaL or US-1, both subtype B) or with medium was extracted using cell lysis buffer (10 mM Tris-HCl [pH8], 1 mM EDTA, 0.001% Triton X-100, 0.001% SDS, and freshly added 1 μg/ml proteinase K) at 30 μl per 1 × 106 cells, followed by successive incubations at 60°C and 95°C for 60 min and 15 min, respectively. Relative multiplex DNA target levels were determined using 2X TaqMan Universal PCR Master Mix and the 7500 Real Time PCR System (Applied Biosystems). Briefly, samples from both a short BaL virus infection time course (0.25–0.5 h) composed of 3 donors in triplicate and a long US-1 virus infection time course (1–96 h) composed of 2 donors in duplicate were processed in 30 μl volumes inclusive of 1 μl of neat lysate. Two primer/probe (dual 5′ FAM- or VIC- and 3′ TAMRA-labeled) sets, differing only in the 3′ primer, were used to differentiate between HIV entry and replication: 1) HIV strong stop (measure of early reverse transcripts, virus entry): 5′R (US-1) (5′-AACTAGGGAACCCACTGCTTAA), 3′U5 (5′-TGAGGGATCTCTAGTTACCAGAGTCA), and R-probe (5′-FAM-CCTCAATAAAGCTTGCCTTGAGTGCTTCAA-TAMRA); and 2) HIV long terminal repeat–gag (measure of late reverse transcripts, replication): 5′R (US-1), 3′gag (5′-CGAGTCCTGCGTCGAGAGA), and R-probe. Amplifications were multiplexed with an Applied Biosystems–designed GAPDH primer/probe set to both normalize sample inputs and to serve as a template integrity control: GAPDH F (5′-ACCGGGAAGGAAATGAATGG), GAPDH R (5′-GCAGGAGCGCAGGGTTAGT), and GAPDH probe (5′-VIC-ACCGGCAGGCTTTCCTAACGGCT-TAMRA). Final HIV and GAPDH primer/probe concentrations were 100/200 and 75/100 nM, respectively. Cycling parameters were 95°C for 10 min, then 50 cycles at 95°C for 15 s, and 60°C for 1 min. Normalized relative HIV target levels were calculated as log2-transformed fold-differences from respective corresponding M-CSF 0.25 or 0.5 h samples values by 2(−∆∆Ct), where ∆∆Ct = (∆Ct of the sample) − (∆Ct of M-CSF 0.25 or 0.5 h) and ∆Ct = (average Ct of HIV target) – (average Ct of corresponding to GAPDH).
HIV-1–infected MDMs, cultured in M-CSF or GM-CSF media, were harvested at 16 h postinfection and then washed in TE buffer without Ca2+ or Mg2+. Cell lysis buffer (50 μl of 10 mM Tris-HCl containing 0.5% Nonidet P-40, 0.5% Tween 20, and 0.3 μg/ml of proteinase K was added to the cell pellet, and the mixture incubated at 55°C for 1 h to digest the cells and release DNA, and subsequently, at 85°C for 15 min to inactivate the proteinase K. An envelope gp120 nested-PCR strategy was performed on the cell lysates at dilutions that yielded 20–30% positive PCR products according to the SGA strategy . The gp120 V1-V4 region nucleotide sequence corresponding to HXB2 number 6354–7485 was retrieved using SGA and sequencing. The number of sequences obtained from the virus stock, purified viruses, and GM-CSF and M-CSF cultured MDMs were 22, 21, 24, and 24, respectively.
Phylogenetic analysis was performed with MEGA 5.05 software to construct a maximum-likelihood tree with the bootstrap method at 100 replications and to calculate the mean pairwise distances. Visual inspections of the distances were also performed for evidence of the signature nucleotides between sequences from different sources. Compartmentalization of the sequences from 4 different sources was evaluated by FST, SM, and AI tests using the software package HyPhy (version 2.10). P < 0.05 (for FST and SM) and bootstrap values >0.95 were considered statistically significant evidence of compartmentalization. For AI, 0 indicates maximum phylogenetic structure, and 1 indicates panmixia.
DNA PCR and sequencing
The first round of PCR reagents was prepared at a final volume of 20 μl per reaction containing 10 μl of 2X GeneAMP Fast PCR Master Mix, 0.5 μl (20 mM) of the first-round primers (gp120, 5′-AGCAGAAGACAGTGGCAATGA; gp120, -3′-AGTGCTTCCTGCTGCTCC), 4 μl of water, and 5 μl of cell lysate. Forty PCR cycles (94°C for 15 s, 58°C for 30 s, and 72°C for 2 min) were performed after denaturing the DNA at 94°C for 1 min. The first-round PCR product (1 μl) was added to 19 μl of the second-round master mix, which included 10 μl of 2X GeneAMP Fast PCR Master Mix, 0.5 μl (20 mM) of the second-round primers (Z1F-TGGGTCACAGTCTATTATGGGGTACCT and JL107-GCTTTTCCTACTTCCTGCCAC) and 8 μl of water. The reaction was performed using the same cycling conditions as described for the first-round amplification. The PCR product was visualized on a 1% agarose gel containing ethidium bromide.
Statistical analysis for cell-surface molecules and HIV-1 infection of MDM was carried out using the Mann-Whitney test (GraphPad Prism 5, version 5.0c; GraphPad Software, La Jolla, CA, USA). A 2-tailed paired t test was used for strong stop (HIV entry) and LTR-gag (HIV replication) experiments. The unpaired t test was used for gag RNA (HIV entry) experiments. P ≤ 0.05 was considered statistically significant.
M-CSF–derived MDMs show greater permissivity to HIV-1 infection
To evaluate the effects of GM-CSF and M-CSF on HIV-1 replication, enriched monocytes from a healthy HIV-seronegative donor were differentiated into MDMs for 5 d in media supplemented with GM-CSF (GM-CSF–derived MDM) or M-CSF (M-CSF–derived MDM) and infected with HIV-1 (US-1, R5 tropic, subtype B). The percentages of infected MDMs were compared by flow cytometry on days 2, 4, and 10 postinfection (Fig. 1A). Because CD14 was down-regulated on GM-CSF–derived MDMs, infected MDMs were expressed as p24+ cells within the gated CD11b population. At day 2 postinfection, there were no significant differences in the percentages of infected MDMs in either the GM-CSF– or the M-CSF–derived MDMs. As infection progressed (day 4 and day 10), intracellular p24 in M-CSF–derived cells was significantly greater than in the GM-CSF–derived MDMs (Fig. 1A). The mean percentage of infected cells at day 4 postinfection was 2.26% in the GM-CSF–derived MDM compared with 12.52% in the M-CSF–derived MDMs (P < 0.002). This difference was even greater on day 10 postinfection (6.70% vs. 57.09%, P < 0.002), respectively. This reflected significantly higher HIV-1 replication in M-CSF compared with GM-CSF–derived MDMs between days 2 and 10 postinfection, respectively (102-fold vs. 12-fold). To further determine whether this observation was virus and/or donor restricted, GM-CSF– and M-CSF–derived MDMs from 6 different donors were infected with 2 additional HIV-1 primary viruses—BaL (subtype B) and MO66 (subtype A/E)—and analyzed on day 4 postinfection (Fig. 1B). Although different degrees of infectivity were observed with the 6 donors, a significantly higher percentage of infected MDMs was observed in the M-CSF–derived MDMs from all 6 donors and with all 3 viruses. Therefore, these data indicated that HIV-1 replication was increased in M-CSF– vs. GM-CSF–derived MDMs and was independent of virus or donor.
HIV-1 entry and replication is significantly decreased in GM-CSF–derived, compared with M-CSF–derived, MDMs.
GM-CSF– or M-CSF–derived MDMs (filled or open bars), were infected with BaL (A and B) or US-1 (C and D). (A) RNA from the lysates at 0.25 and 0.5 h postinfection was subjected to qRT-PCR for HIV-1 gag RNA (virus entry). ΔCt values for HIV gag RNA was normalized to GAPDH RNA. A lower ΔCt value indicates a higher amount of viral RNA or higher viral entry. Lysates collected at 0.25, 0.5, 1, 3, 24, and 96 h postinfection were analyzed for early strong stop or late LTR-gag DNA reverse transcripts indicative of virus entry (B, C) or replication (D). GAPDH-normalized target levels are displayed relative to the corresponding 0.25 h sample for (B) or to 1 h samples (C and D) of M-CSF differentiated MDM as mean log2-transformed fold-change ± S.D. for either 3 or 2 iterations of 3 or 2 independent experiments (B). (*P = 0.01; **P = 0.001; ***P = 0.0001; 2-tailed paired t tests).
Independent early time courses, harvested at 0.25 and 0.5 h time points, were assessed for HIV strong stops. HIV-1 strong-stop targets, indicative of virus entry, were detected by the presence of R-U5 LTR cDNA sequences, whereas 2-LTR targets, indicative of virus replication, were detected by the presence of LTR-gag DNA sequences. GAPDH-normalized target levels are displayed, relative to those of the M-CSF differentiated MDMs, at the corresponding 0.25 h sample as log2-transformed fold-change means ± SD for 3 iterations of 3 experiments (Fig. 2B). Sample sets were compared using 2-tailed paired t tests (***P = 0.0001). Both 0.25 and 0.5 h time points showed increased strong stop targets over time in both MDM populations, with significantly less target detection in the GM-CSF–derived MDMs. Fold-differences in detected strong stop targets between GM-CSF– and M-CSF–derived MDMs were 7.1 and 6.9, respectively (Fig. 2B). The 2-LTR HIV-1 DNA targets were not consistently detected, indicating that either no HIV-1 replication occurred that early or that the levels were below detection. The uninfected HIV− controls produced no strong stop amplification signals.
In separate experiments, lysates were collected at 1, 3, 24, and 96 h postinfection and subjected to relative qPCR analysis to detect early strong-stop reverse transcripts (Fig. 2C) and late LTR-gag DNA reverse transcripts (Fig. 2D). Mock infection samples were negative through the 96-h time point (data not shown). GAPDH-normalized target levels are displayed relative to those of the M-CSF–differentiated MDMs at the corresponding 1 h samples as log2-transformed fold-change means ± SD for 2 iterations from each set of 2 independent experiments (Fig. 2C and D). Sample sets were compared using 2-tailed paired t tests (*P = 0.01, **P = 0.001). HIV-1 strong-stop targets increased steadily in both MDM populations, but were significantly lower in the GM-CSF–derived MDMs at all time-points (Fig. 2C). Differences in detected strong-stop DNA between the GM-CSF– and M-CSF–derived MDMs at 1, 3, 24, and 96 h time points were 15.1-, 5.6-, 2.7-, and 99.6-fold, respectively (Fig. 2C). Correspondingly, the presence of 2-LTR HIV-1 DNA, which is indicative of virus replication, increased steadily in both MDM populations. However, at the later time points (24 h and 96 h) postinfection, it was significantly less in the GM-CSF–, compared with the M-CSF–derived, MDMs (P < 0.001). Differences for 2-LTR HIV-1 levels between the GM-CSF– and M-CSF–derived MDMs at 1, 3, 24, and 96 h were 4.2-, 0.6-, 8.2-, and 93.5-fold, respectively (Fig. 2D). The uninfected HIV− controls produced no HIV-1 RNA gag signals. Collectively, these data suggest that the significantly decreased level of HIV-1 replication in the GM-CSF–derived MDMs may be the result of reduced HIV-1 entry. In each case, sample sets were compared using 2-tailed paired t tests (*P = 0.01, **P = 0.001, ***P = 0.0001).
GM-CSF–derived MDMs express fewer Siglec-1 receptors
The role of CD4  and chemokine receptors  as cell-surface receptors for HIV-1 entry into target cells has been extensively documented. The differences in HIV-1 replication between GM-CSF–derived MDMs and their M-CSF–derived counterparts may be largely due to differential expression of cell-surface receptors used by HIV-1 to enter into target cells. Therefore, we evaluated the cell-surface expression of HIV-1 receptors, Fcγ receptors, antigen-presenting molecules, integrin receptors and myeloid markers, costimulatory molecules, and Siglec receptors on uninfected MDMs from 4 donors on day 5 after in vitro differentiation with GM-CSF and M-CSF. Representative histogram plots of 1 donor are shown in Fig. 3, and the average of the MFIs of the 4 donor cells is shown in Table 1. Minor differences were noted in the MFIs of CD4, CCR5, antigen-presenting molecules, costimulatory molecules, and Siglec-9 receptors on M-CSF–derived MDMs compared with GM-CSF–derived MDMs. Although CD4 expression was detected in both MDM populations, this was not due to contamination of T cells, as shown by the absence of CD3 staining (data not shown). Slight differences in the surface expression of DC-SIGN, CD64, CD11b, Siglec-3, Siglec-5, and CD32 were observed in the M-CSF–. compared with GM-CSF–derived MDMs. In contrast, there was a 5.5-fold increase in the expression of Siglec-1 (CD169) receptors in the presence of M-CSF compared with GM-CSF. Recently, the importance of Siglec-1 in HIV-1 capture and transmission by DCs [13, 17] and their ability to facilitate HIV-1 infection of macrophages  has been described. Therefore, we quantified the number of Siglec-1 receptors/cell on GM-CSF– and M-CSF–derived MDMs in 3 donors. In addition, we also performed similar analyses for CCR-5 and CD4 receptors. As shown in Table 2, the mean number of Siglec-1, CCR-5, and CD4 receptors was lower in the GM-CSF– compared with the M-CSF–derived MDMs.
|Surface molecule||M-CSF–derived MDMs||GM-CSF–derived MDMs|
|CD4||7.20 ± 0.67||6.15 ± 0.26|
|CCR5||8.51 ± 0.59||10.11 ± 0.65|
|DC-SIGN||7.12 ± 0.49||3.05 ± 0.69|
|CD16||13.90 ± 1.38||3.61 ± 0.51|
|CD32||258.00 ± 65.15||178.10 ± 73.48|
|CD64||15.81 ± 0.72||6.45 ± 0.72|
|CD40||12.72 ± 1.39||18.11 + 1.70|
|CD80||10.15 ± 0.74||11.80 ± 0.62|
|CD86||98.51 ± 1.16||112.93 ± 5.08|
|MHC I||301.10 ± 13.11||265.51 ± 11.12|
|MHC II||490.82 ± 16.93||465.54 ± 12.35|
|CD1a||10.25 ± 1.91||8.83 ± 0.82|
|CD11b||44.95 ± 5.42||83.53 ± 4.47|
|CD14||79.15 ± 12.11||16.48 ± 2.13|
|Siglec-1||88.83 ± 9.57||16.28 ± 4.64|
|Siglec-3||86.00 ± 6.14||23.10 ± 4.24|
|Siglec-5||15.83 ± 6.51||8.80 ± 1.31|
|Siglec-9||51.83 ± 7.20||47.05 ± 4.68|
The MFI of cell-surface markers was determined by flow cytometry. Values are from triplicate samples (means ± sd) from M-CSF– and GM-CSF–derived MDMs from 4 different donors.
|Surface molecule||Donor No.||M-CSF–derived MDM||GM-CSF–derived MDM|
|Siglec-1||124||67,879 ± 5,856||10,872 ± 3,286|
|130||79,190 ± 1,011||33,084 ± 824|
|202||32,966 ± 6,536||14,479 ± 469|
|CCR-5||124||70,601 ± 5,784||14,902 ± 614|
|130||29,690 ± 350||14,021 ± 277|
|202||29,861 ± 1,067||25,542 ± 419|
|CD4||124||92,719 ± 3,523||38,034 ± 1,158|
|130||68,064 ± 3,220||50,286 ± 1,639|
|202||36,458 ± 1,322||30,808 ± 1,556|
The number of cell-surface molecules was determined by flow cytometry. Values are from triplicate samples (means ± sd) from M-CSF– and GM-CSF–derived MDMs from 3 different donors.
Siglec-1 binds HIV-1 and facilitates entry and infectivity of GM-CSF– and M-CSF–derived MDMs
To evaluate the involvement of the various Siglec receptors in HIV-1 infection, GM-CSF– and M-CSF–derived MDMs from 2 donors (donors 130 and 132) were preincubated with purified mAbs to block Siglec-1, -3, -5, or -9 and then infected with HIV-1 (US-1, subtype B). Consistently, in both donors (donors 130, Fig. 4A; and 132, Fig. 4B; dot plots and their respective bar graphs), blocking Siglec-1 significantly decreased HIV-1 infection in both GM-CSF– (P = 0.008) and M-CSF–derived MDMs (P = 0.007) by 80–97%. Blocking the other Siglec molecules (Siglec-3, -5, or -9) resulted in 5–28% and 12–48% reduction of HIV-1 infection for M-CSF– and GM-CSF–derived MDMs, respectively (Fig. 4A and B). These results indicate that, compared with the other Siglec molecules, Siglec-1 was the predominant receptor involved in MDM/HIV interactions.
Primary human monocytes were differentiated into MDM following in vitro culture in GM-CSF or M-CSF media. Triplicate wells of MDMs from donor 130 (A) and donor 132 (B) were preincubated with purified mAbs specific for the Siglec receptors (Siglec-1, -3, -5, or -9) and subsequently infected with US-1 (5 ng p24). MDMs preincubated with PBS served as infection controls. Cells were harvested on day 4 postinfection and analyzed for the presence of intracellular p24 by flow cytometry. Panels show dot plots of US-1–infected GM-CSF– or M-CSF–derived MDMs. Values in the upper right quadrants represent the percentage of infected MDMs. The data from Figs. 4A and B are represented as bar graphs (right side), and shows the percentage inhibition of infectivity (means ± sd) in GM-CSF–derived MDMs (filled bars) or in M-CSF–derived MDMs (open bars). Data are representative of triplicate wells from 3 independent experiments.
To further compare the involvement of Siglec-1 and other well-characterized HIV-1 receptors (CD4 and CCR5 molecules) involved in HIV-1 infection, M-CSF–derived MDMs from 3 donors (donors 132, 130, and 202) were evaluated for HIV-1 infectivity (Fig. 5A). In all 3 donors, blocking Siglec-1 receptor decreased HIV-1 infection by 84–94% (P = 0007). In all 3 donors, blocking the CD4 receptor resulted in a 50–56% decrease in HIV-1 infectivity. In contrast, blocking the CCR5 receptor resulted in only a minimal reduction in HIV-1 infectivity in all 3 donors (Fig. 5A).
V1V2 protein binds specifically to Siglec-1.
(A) Triplicate wells of M-CSF–derived MDMs were preincubated with recombinant CHO-expressed Soc–V1V2Zm249 (CHO) envelope protein, Soc (CHO), or PBS for 30 min at room temperature, and the expression of Siglec-1, Siglec-9, and CD4 was analyzed by flow cytometry. Bar graphs show percentage of expression (means ± sd) of the cell receptors. A representative of 2 independent experiments is shown (*P = 0.02, Mann Whitney test). (B) Siglec-1 was immobilized on a CM5 biosensor chip and the binding (RUs) of CHO-expressed Soc (CHO), Soc-V1V2Zm249 (CHO), or 293F-expressed Soc-V1V2JRFL (293F) recombinant Soc–V1V2 proteins was determined by SPR. (C) MDMs were preincubated with PBS or with recombinant Soc-V1V2 proteins for 30 min at room temperature and infected with HIV-1. Cells were harvested on day 4 postinfection and analyzed for the presence of intracellular p24 by flow cytometry. Bar graphs show the percentage inhibition of infectivity (means ± sd). Data are representative of 2 independent experiments.
The interaction of the V1V2 proteins with Siglec-1 was further evaluated using an SPR assay (Fig. 7B, and Supplemental Table 1). Three different V1V2 proteins (transmitted/founder subtype C Soc-V1V2Zm249, subtype B Soc-V1V2WITO, and subtype B Soc-V1V2JR-FL) showed significantly more binding to immobilized Siglec-1 compared with the Soc protein alone. The extremely low level of binding by Soc alone to Siglec-1 established the specificity of binding and the interaction through the V1V2 region (Fig. 7B and Supplemental Table 1). Although the glycosylation patterns of the CHO and 293F proteins may differ, they did not exhibit any significant differences in binding to Siglec-1. Synthetic, nonglycosylated, linear and cyclic V2 peptides did not bind to immobilized Siglec-1 (data not shown), thus signifying the importance of glycans and/or protein conformation during the binding interactions.
To determine whether binding of the recombinant V1V2 envelope proteins to Siglec-1 would modulate HIV-1 infection, MDMs were preincubated with Soc-V1V2Zm249 (CHO), Soc-V1V2JR-FL (293F), Soc (CHO-S), or PBS and were subsequently infected with US-1. As shown in Fig. 7C, preincubation of MDMs with either of the V1V2 recombinant proteins resulted in a 45% decrease in viral infection when compared with MDMs preincubated with Soc or PBS.
Siglec-1 binds to HIV envelope proteins and to the V1V2 region with high affinity
The binding affinities of recombinant envelope proteins (293F-expressed monomeric subtype B JR-FL gp120 and trimeric JR-FL and SF162 gp145 with foldon, a trimerization domain from bacteriophage T4 fibritin), and V1V2 proteins to immobilized Siglec-1 (2500 RUs) were determined by SPR (Fig. 8). Kinetic studies were carried out with varying concentrations of the envelope protein. The bivalent model was the best fit for the data, indicating that there could be multiple sites on the envelope protein for binding to Siglec-1 (Fig. 8). These data showed that the recombinant envelope proteins tested were bound to Siglec-1 with low nanomolar affinity. These data showed that the binding affinities for the JR-FL and SF162 gp145 trimeric proteins to immobilized Siglec-1 (Fig. 8B and 8C) were similar and were 9- to 15-fold higher than the binding affinity to monomeric gp120 protein (Fig. 8A). In the reverse experiment, Siglec-1 did not bind to the immobilized monomeric envelope proteins. However, it bound to immobilized trimeric proteins with a 10- to 20-fold less affinity than the binding of trimeric proteins to immobilized Siglec-1 (compare Fig. 8B and C with D and E). We then determined the binding affinities of Soc-V1V2JR-FL (293F) and Soc-V1V2SF162 (CHO-S) to immobilized Siglec-1. As shown in Fig. 8F and G, Soc-V1V2JR-FL (293F) and Soc-V1V2SF162 (CHO-S) bound to Siglec-1 with much less affinity (15.5-fold and 4.5-fold, respectively) compared with the corresponding trimeric proteins. These data suggest that there might be multiple sialyl binding sites for Siglec-1 on the envelope proteins.
Sialic acid inhibits binding of monomeric and trimeric HIV-1 envelope proteins and V1V2 to Siglec-1.
JR-FL FD trimeric gp145 and SF162 FD trimeric gp145 (50 nM) proteins (A) or Soc-V1V2JR-FL and Soc-V1V2SF162 (100 nM) (B) were incubated in the absence or presence of 6′-sialyllactose (SL, 1 mM), SA (SA, 10 mM), or lactose (L, 10 mM) and were then injected over immobilized Siglec-1 (3700 RUs). In separate experiments, Siglec-1 (50 nM) was incubated in the absence or presence of 6′-sialyllactose (SL, 10 mM), SA (SA, 10 mM), or lactose (L, 10 mM) and then injected over immobilized JR-FL FD trimeric gp145 (1400 RUs) or SF162 FD trimeric gp145 (900 RU) (C) or JR-FL gp120 (D) or Soc-V1V2JR-FL (E). The data are represented as bar graphs and show the percentage binding (means ± sd) of triplicate measurements as determined by SPR analysis.
Soc–V1V2 protein inhibits HIV-1 entry and replication
Based on the above results, we next determined whether gp145 trimers and Soc-V1V2Zm249 protein would inhibit virus entry. As shown in Supplemental Fig. 2, JR-FL gp145 trimer inhibited the entry of BaL into M-CSF–derived MDMs in a dose-dependent manner in the 2 donors tested. Similarly, varying concentrations of Soc-V1V2Zm249 protein also inhibited the entry of BaL, with 60% inhibition observed at a concentration of 20 μg/ml of Soc-V1V2Zm249 (Fig. 10A). This optimum concentration was then evaluated in a subsequent experiment in 3 donors. In contrast to the Soc protein (20 μg/ml), the Soc-V1V2Zm249 protein (20 μg/ml) significantly inhibited, to varying levels, the entry of BaL into M-CSF–derived MDMs in all 3 donors (Fig. 10B).
O.J., H.V.T., and M.R. conceived and designed the experiments. O.J., H.V.T., J.K., W.A., P.K.E., and K.K.P. performed the experiments. O.J., H.V.T., P.K.E., S.T., and V.B.R. analyzed the data. O.J., H.V.T., J.H.K, N.L.M., C.R.A., V.B.R., and M.R.. discussed the data. W.A., G.G., R.T., and V.B.R contributed reagents/materials/analysis tools or provided helpful suggestions. O.J. and M.R. wrote the paper with contributions from all authors.
The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Army, Department of Defense, nor the U.S. Government.
H.V.T. was partially supported by a grant from the Swiss National Science Foundation. V.B.R. was partially supported by the U.S. National Institutes of Health National Institute of Allergy and Infectious Diseases (Grant AI102725). This work was supported by a cooperative agreement (W81XWH-11-2-0174) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Department of Defense. We thank Kultida Poltavee for sequence analysis, Gustavo Kijak and Peter Phuc Pham for their assistance with phylogenetic analysis, Agnes-Laurence Chenine for providing the envelope-deficient HIV-1 (pSG3Δenv), and Sayali Onkar for her assistance in generating MDMs.
|AI||Simmonds Association Index|
|CHO||Chinese hamster ovary|
|FST||Wright’s measure of population subdivision|
|LTR||long terminal repeat|
|M-CSF||macrophage colony-stimulating factor|
|MFI||mean fluorescent intensity|
|PNGase F||peptide N-linked glycosidase F|
|qPCR||quantitative polymerase chain reaction|
|qRT-PCR||quantitative reverse transcriptase-polymerase chain reaction|
|Siglec||Sialic acid-binding immunoglobulin-like lectin|
|Soc||small outer capsid protein|
|SPR||surface plasmon resonance|
|WRAIR||Walter Reed Army Institute of Research|
The online version of this paper, found at www.jleukbio.org, includes supplemental information.
The authors declare no conflicts of interest.
- Strauss-Ayali D., Conrad S. M., Mosser D. M. (2007) Monocyte subpopulations and their differentiation patterns during infection.J. Leukoc. Biol. 82, 244–252. [PubMed] [Google Scholar]
- Gordon S., Taylor P. R. (2005) Monocyte and macrophage heterogeneity.Nat. Rev. Immunol. 5, 953–964. [PubMed] [Google Scholar]
- Mantovani A., Sica A., Sozzani S., Allavena P., Vecchi A., Locati M. (2004) The chemokine system in diverse forms of macrophage activation and polarization.Trends Immunol. 25, 677–686. [PubMed] [Google Scholar]
- Stout R. D., Suttles J. (2004) Functional plasticity of macrophages: reversible adaptation to changing microenvironments.J. Leukoc. Biol. 76, 509–513. [PMC free article] [PubMed] [Google Scholar]
- Abumaree M. H., Al Jumah M. A., Kalionis B., Jawdat D., Al Khaldi A., Abomaray F. M., Fatani A. S., Chamley L. W., Knawy B. A. (2013) Human placental mesenchymal stem cells (pMSCs) play a role as immune suppressive cells by shifting macrophage differentiation from inflammatory M1 to anti-inflammatory M2 macrophages.Stem Cell Rev. 9, 620–641. [PubMed] [Google Scholar]
- Verreck F. A., de Boer T., Langenberg D. M., Hoeve M. A., Kramer M., Vaisberg E., Kastelein R., Kolk A., de Waal-Malefyt R., Ottenhoff T. H. (2004) Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria.Proc. Natl. Acad. Sci. U. S. A. 101, 4560–4565. [PMC free article] [PubMed] [Google Scholar]
- Mills C. D., Kincaid K., Alt J. M., Heilman M. J., Hill A. M. (2000) M-1/M-2 macrophages and the Th1/Th2 paradigm.J. Immunol. 164, 6166–6173. [PubMed] [Google Scholar]
- Mantovani A., Sica A., Locati M. (2005) Macrophage polarization comes of age.Immunity 23, 344–346. [PubMed] [Google Scholar]
- Cicala C., Arthos J., Fauci A. S. (2011) HIV-1 envelope, integrins and co-receptor use in mucosal transmission of HIV.J. Transl. Med. 9(Suppl 1), S2. [PMC free article] [PubMed] [Google Scholar]
- McKinnon L. R., Nyanga B., Chege D., Izulla P., Kimani M., Huibner S., Gelmon L., Block K. E., Cicala C., Anzala A. O., Arthos J., Kimani J., Kaul R. (2011) Characterization of a human cervical CD4+ T cell subset coexpressing multiple markers of HIV susceptibility.J. Immunol. 187, 6032–6042. [PubMed] [Google Scholar]
- Von Lindern J. J., Rojo D., Grovit-Ferbas K., Yeramian C., Deng C., Herbein G., Ferguson M. R., Pappas T. C., Decker J. M., Singh A., Collman R. G., O’Brien W. A. (2003) Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages.J. Virol. 77, 3624–3633. [PMC free article] [PubMed] [Google Scholar]
- Saphire A. C., Bobardt M. D., Zhang Z., David G., Gallay P. A. (2001) Syndecans serve as attachment receptors for human immunodeficiency virus type 1 on macrophages.J. Virol. 75, 9187–9200. [PMC free article] [PubMed] [Google Scholar]
- Puryear W. B., Akiyama H., Geer S. D., Ramirez N. P., Yu X., Reinhard B. M., Gummuluru S. (2013) Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.PLoS Pathog. 9, e1003291. [PMC free article] [PubMed] [Google Scholar]
- Izquierdo-Useros N., Lorizate M., McLaren P. J., Telenti A., Kräusslich H. G., Martinez-Picado J. (2014) HIV-1 capture and transmission by dendritic cells: the role of viral glycolipids and the cellular receptor Siglec-1.PLoS Pathog. 10, e1004146. [PMC free article] [PubMed] [Google Scholar]
- Zou Z., Chastain A., Moir S., Ford J., Trandem K., Martinelli E., Cicala C., Crocker P., Arthos J., Sun P. D. (2011) Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.PLoS One 6, e24559. [PMC free article] [PubMed] [Google Scholar]
- Crocker P. R., Paulson J. C., Varki A. (2007) Siglecs and their roles in the immune system.Nat. Rev. Immunol. 7, 255–266. [PubMed] [Google Scholar]
- Izquierdo-Useros N., Lorizate M., Puertas M. C., Rodriguez-Plata M. T., Zangger N., Erikson E., Pino M., Erkizia I., Glass B., Clotet B., Keppler O. T., Telenti A., Kräusslich H. G., Martinez-Picado J. (2012) Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.PLoS Biol. 10, e1001448. [PMC free article] [PubMed] [Google Scholar]
- Karasavvas N., Billings E., Rao M., Williams C., Zolla-Pazner S., Bailer R. T., Koup R. A., Madnote S., Arworn D., Shen X., Tomaras G. D., Currier J. R., Jiang M., Magaret C., Andrews C., Gottardo R., Gilbert P., Cardozo T. J., Rerks-Ngarm S., Nitayaphan S., Pitisuttithum P., Kaewkungwal J., Paris R., Greene K., Gao H., Gurunathan S., Tartaglia J., Sinangil F., Korber B. T., Montefiori D. C., Mascola J. R., Robb M. L., Haynes B. F., Ngauy V., Michael N. L., Kim J. H., de Souza M. S.; MOPH TAVEG Collaboration (2012) The Thai Phase III HIV Type 1 Vaccine trial (RV144) regimen induces antibodies that target conserved regions within the V2 loop of gp120.AIDS Res. Hum. Retroviruses 28, 1444–1457. [PMC free article] [PubMed] [Google Scholar]
- Sun S., Gao S., Kondabagil K., Xiang Y., Rossmann M. G., Rao V. B. (2012) Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages.Proc. Natl. Acad. Sci. U. S. A. 109, 817–822. [PMC free article] [PubMed] [Google Scholar]
- Stemmer W. P., Crameri A., Ha K. D., Brennan T. M., Heyneker H. L. (1995) Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.Gene 164, 49–53. [PubMed] [Google Scholar]
- Yang X., Lee J., Mahony E. M., Kwong P. D., Wyatt R., Sodroski J. (2002) Highly stable trimers formed by human immunodeficiency virus type 1 envelope glycoproteins fused with the trimeric motif of T4 bacteriophage fibritin.J. Virol. 76, 4634–4642. [PMC free article] [PubMed] [Google Scholar]
- Jobe O., Peachman K. K., Matyas G. R., Asher L. V., Alving C. R., Rao M. (2012) An anti-phosphoinositide-specific monoclonal antibody that neutralizes HIV-1 infection of human monocyte-derived macrophages.Virology 430, 110–119. [PubMed] [Google Scholar]
- Salazar-Gonzalez J. F., Bailes E., Pham K. T., Salazar M. G., Guffey M. B., Keele B. F., Derdeyn C. A., Farmer P., Hunter E., Allen S., Manigart O., Mulenga J., Anderson J. A., Swanstrom R., Haynes B. F., Athreya G. S., Korber B. T., Sharp P. M., Shaw G. M., Hahn B. H. (2008) Deciphering human immunodeficiency virus type 1 transmission and early envelope diversification by single-genome amplification and sequencing.J. Virol. 82, 3952–3970. [PMC free article] [PubMed] [Google Scholar]
- Walter B. L., Wehrly K., Swanstrom R., Platt E., Kabat D., Chesebro B. (2005) Role of low CD4 levels in the influence of human immunodeficiency virus type 1 envelope V1 and V2 regions on entry and spread in macrophages.J. Virol. 79, 4828–4837. [PMC free article] [PubMed] [Google Scholar]
- Tuttle D. L., Harrison J. K., Anders C., Sleasman J. W., Goodenow M. M. (1998) Expression of CCR5 increases during monocyte differentiation and directly mediates macrophage susceptibility to infection by human immunodeficiency virus type 1.J. Virol. 72, 4962–4969. [PMC free article] [PubMed] [Google Scholar]
- Leonard C. K., Spellman M. W., Riddle L., Harris R. J., Thomas J. N., Gregory T. J. (1990) Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells.J. Biol. Chem. 265, 10373–10382. [PubMed] [Google Scholar]
- Wyatt R., Sodroski J. (1998) The HIV-1 envelope glycoproteins: fusogens, antigens, and immunogens.Science 280, 1884–1888. [PubMed] [Google Scholar]
- Rempel H, Calosing C, Sun B, Pulliam L (2008) Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.PLoS One 3, e1967. [PMC free article] [PubMed] [Google Scholar]
- Fleetwood A. J., Dinh H., Cook A. D., Hertzog P. J., Hamilton J. A. (2009) GM-CSF- and M-CSF-dependent macrophage phenotypes display differential dependence on type I interferon signaling.J. Leukoc. Biol. 86, 411–421. [PubMed] [Google Scholar]
- Cassetta L., Kajaste-Rudnitski A., Coradin T., Saba E., Della Chiara G., Barbagallo M., Graziano F., Alfano M., Cassol E., Vicenzi E., Poli G. (2013) M1 polarization of human monocyte-derived macrophages restricts pre and postintegration steps of HIV-1 replication.AIDS 27, 1847–1856. [PubMed] [Google Scholar]
- Burgess A. W., Metcalf D. (1980) The nature and action of granulocyte-macrophage colony stimulating factors.Blood 56, 947–958. [PubMed] [Google Scholar]
- Hamilton J. A., Stanley E. R., Burgess A. W., Shadduck R. K. (1980) Stimulation of macrophage plasminogen activator activity by colony-stimulating factors.J. Cell. Physiol. 103, 435–445. [PubMed] [Google Scholar]
- Hamilton J. A. (2008) Colony-stimulating factors in inflammation and autoimmunity.Nat. Rev. Immunol. 8, 533–544. [PubMed] [Google Scholar]
- Sorgi C. A., Rose S., Court N., Carlos D., Paula-Silva F. W., Assis P. A., Frantz F. G., Ryffel B., Quesniaux V., Faccioli L. H. (2012) GM-CSF priming drives bone marrow-derived macrophages to a pro-inflammatory pattern and downmodulates PGE2 in response to TLR2 ligands.PLoS One 7, e40523. [PMC free article] [PubMed] [Google Scholar]
- Vogt G., Nathan C. (2011) In vitro differentiation of human macrophages with enhanced antimycobacterial activity.J. Clin. Invest. 121, 3889–3901. [PMC free article] [PubMed] [Google Scholar]
- Kedzierska K., Maerz A., Warby T., Jaworowski A., Chan H., Mak J., Sonza S., Lopez A., Crowe S. (2000) Granulocyte-macrophage colony-stimulating factor inhibits HIV-1 replication in monocyte-derived macrophages.AIDS 14, 1739–1748. [PubMed] [Google Scholar]
- Gruber M. F., Weih K. A., Boone E. J., Smith P. D., Clouse K. A. (1995) Endogenous macrophage CSF production is associated with viral replication in HIV-1-infected human monocyte-derived macrophages.J. Immunol. 154, 5528–5535. [PubMed] [Google Scholar]
- Cassol E., Cassetta L., Rizzi C., Alfano M., Poli G. (2009) M1 and M2a polarization of human monocyte-derived macrophages inhibits HIV-1 replication by distinct mechanisms.J. Immunol. 182, 6237–6246. [PubMed] [Google Scholar]
- Platt E. J., Wehrly K., Kuhmann S. E., Chesebro B., Kabat D. (1998) Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1.J. Virol. 72, 2855–2864. [PMC free article] [PubMed] [Google Scholar]
- Bergamini A., Perno C. F., Dini L., Capozzi M., Pesce C. D., Ventura L., Cappannoli L., Falasca L., Milanese G., Caliò R., et al. (1994) Macrophage colony-stimulating factor enhances the susceptibility of macrophages to infection by human immunodeficiency virus and reduces the activity of compounds that inhibit virus binding.Blood 84, 3405–3412. [PubMed] [Google Scholar]
- Kutza J., Fields K., Grimm T. A., Clouse K. A. (2002) Inhibition of HIV replication and macrophage colony-stimulating factor production in human macrophages by antiretroviral agents.AIDS Res. Hum. Retroviruses 18, 619–625. [PubMed] [Google Scholar]
- Van der Kuyl A. C., van den Burg R., Zorgdrager F., Groot F., Berkhout B., Cornelissen M. (2007) Sialoadhesin (CD169) expression in CD14+ cells is upregulated early after HIV-1 infection and increases during disease progression.PLoS One 2, e257. [PMC free article] [PubMed] [Google Scholar]
- Pulliam L., Sun B., Rempel H. (2004) Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load.J. Neuroimmunol. 157, 93–98. [PubMed] [Google Scholar]
- Pino M., Erkizia I., Benet S., Erikson E., Fernández-Figueras M. T., Guerrero D., Dalmau J., Ouchi D., Rausell A., Ciuffi A., Keppler O. T., Telenti A., Kräusslich H. G., Martinez-Picado J., Izquierdo-Useros N. (2015) HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology 12, 37. [PMC free article] [PubMed] [Google Scholar]
- Hartnell A., Steel J., Turley H., Jones M., Jackson D. G., Crocker P. R. (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.Blood 97, 288–296. [PubMed] [Google Scholar]
- Munday J., Floyd H., Crocker P. R. (1999) Sialic acid binding receptors (siglecs) expressed by macrophages.J. Leukoc. Biol. 66, 705–711. [PubMed] [Google Scholar]
- Van Montfort T., Eggink D., Boot M., Tuen M., Hioe C. E., Berkhout B., Sanders R. W. (2011) HIV-1 N-glycan composition governs a balance between dendritic cell-mediated viral transmission and antigen presentation.J. Immunol. 187, 4676–4685. [PMC free article] [PubMed] [Google Scholar]
- Shen R, Raska M, Bimczok D, Novak J, Smith PD (2014) HIV-1 envelope glycan moieties modulate HIV-1 transmission.J. Virol. 88, 14258–14267. [PMC free article] [PubMed] [Google Scholar]
- Izquierdo-Useros N., Lorizate M., Contreras F. X., Rodriguez-Plata M. T., Glass B., Erkizia I., Prado J. G., Casas J., Fabriàs G., Kräusslich H. G., Martinez-Picado J. (2012) Sialyllactose in viral membrane gangliosides is a novel molecular recognition pattern for mature dendritic cell capture of HIV-1.PLoS Biol. 10, e1001315. [PMC free article] [PubMed] [Google Scholar]
- Doores K. J., Burton D. R. (2010) Variable loop glycan dependency of the broad and potent HIV-1-neutralizing antibodies PG9 and PG16.J. Virol. 84, 10510–10521. [PMC free article] [PubMed] [Google Scholar]
- McLellan J. S., Pancera M., Carrico C., Gorman J., Julien J. P., Khayat R., Louder R., Pejchal R., Sastry M., Dai K., O’Dell S., Patel N., Shahzad-ul-Hussan S., Yang Y., Zhang B., Zhou T., Zhu J., Boyington J. C., Chuang G. Y., Diwanji D., Georgiev I., Kwon Y. D., Lee D., Louder M. K., Moquin S., Schmidt S. D., Yang Z. Y., Bonsignori M., Crump J. A., Kapiga S. H., Sam N. E., Haynes B. F., Burton D. R., Koff W. C., Walker L. M., Phogat S., Wyatt R., Orwenyo J., Wang L. X., Arthos J., Bewley C. A., Mascola J. R., Nabel G. J., Schief W. R., Ward A. B., Wilson I. A., Kwong P. D. (2011) Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.Nature 480, 336–343. [PMC free article] [PubMed] [Google Scholar]
- Amin M. N., McLellan J. S., Huang W., Orwenyo J., Burton D. R., Koff W. C., Kwong P. D., Wang L. X. (2013) Synthetic glycopeptides reveal the glycan specificity of HIV-neutralizing antibodies.Nat. Chem. Biol. 9, 521–526. [PMC free article] [PubMed] [Google Scholar]
- Julien J. P., Lee J. H., Cupo A., Murin C. D., Derking R., Hoffenberg S., Caulfield M. J., King C. R., Marozsan A. J., Klasse P. J., Sanders R. W., Moore J. P., Wilson I. A., Ward A. B. (2013) Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9.Proc. Natl. Acad. Sci. U. S. A. 110, 4351–4356. [PMC free article] [PubMed] [Google Scholar]
- Klaas M., Oetke C., Lewis L. E., Erwig L. P., Heikema A. P., Easton A., Willison H. J., Crocker P. R. (2012) Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni.J. Immunol. 189, 2414–2422. [PMC free article] [PubMed] [Google Scholar]
Articles from Journal of Leukocyte Biology are provided here courtesy of The Society for Leukocyte Biology