rgp41 HIV-1

$435.00
SKU: 1091
Size: 100 ug

SPECIFICATIONS                                                                     APPLICATIONS

  Concentration:            See vial                                               In-Vitro Diagnostics

  Mass/vial:                   100ug                                                  Immunization

  Volume/vial:               see vial                                                 T-Cell Activation

  Diluent:                      10 mM Tris, pH8,0.002%SDS                                                      

  Purity:                        >98%                                                                           

  Stabilizer:                  None                                                       

  Preservative:             None                                                    

  Storage:                     -75oC

  Physical State:          Frozen Liquid

  Stability:                    1 year at -20oC, 3 years at -75oC.  

                                   

DESCRIPTION: Truncated Recombinant HIV-1 MN envelope protein gp41 produced in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.

PURIFICATION: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.

BIOLOGICAL ACTIVITY:  Not done

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.

Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.