Item# 1091 Recombiant protein gp41 HIV-1(E.coli)
Concentration: See vial In-Vitro
Mass/vial: 100ug Immunization
Volume/vial: see vial T-Cell Activation
Diluent: 10 mM Tris, pH8,0.002%SDS Diagnostics
Physical State: Frozen Liquid
Stability: 1 year at -20oC, 3 years at -75oC.
DESCRIPTION: Truncated Recombinant HIV-1 MN envelope protein gp41 produced in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.
PURIFICATION: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.
BIOLOGICAL ACTIVITY: Not done
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.
Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.