rgp41 HIV-1(E.coli)

ImmunoDXSKU: 1091

Price:
$435

Description

PRODUCT SPECIFICATION

Item# 1091 Recombiant protein gp41 HIV-1(E.coli)

SPECIFICATIONS                                  APPLICATIONS

  Concentration:            See vial                                     In-Vitro       

  Mass/vial: 100ug                                                           Immunization

 Volume/vial: see vial                                                       T-Cell Activation

  Diluent: 10 mM Tris, pH8,0.002%SDS                          Diagnostics                       

  Purity:                        >98%                                                                           

  Stabilizer:                  None                                                       

  Preservative:             None                                                    

  Storage:                     -75oC

  Physical State:          Frozen Liquid

  Stability:                    1 year at -20oC, 3 years at -75oC.  

                                   

DESCRIPTION: Truncated Recombinant HIV-1 MN envelope protein gp41 produced in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.

PURIFICATION: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.

BIOLOGICAL ACTIVITY:  Not done

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.

Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.

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